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Image Search Results
Journal: Nature Cell Biology
Article Title: The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness
doi: 10.1038/s41556-022-00925-9
Figure Lengend Snippet: a , Proteins that interact with NLS1–HK2, as determined by BioID coupled with mass spectrometry; n = 3 biological repeats. b , Intensity of the PLA signal of endogenous HK2 and MAX, SIRT1, IWS1, CTR9 and SPIN1 in NB4 cells. A.u., arbitrary units; n = 278 cells from three biological repeats. c , Chromatin accessibility, measured through ATAC-seq, following overexpression of NLS1–HK2 in NB4 cells; n = 3 biological repeats. d , LSC + and LSC − signatures in EV control NB4 cells. LSC − : minimum, 5.15; maximum, 12.81; and median, 8.27. LSC + : minimum, 5.990; maximum, 12.030; and median, 9.325. e , LSC + and LSC − signatures in NLS1–HK2 NB4 cells. LSC − : minimum, 6.840; maximum, 13.010; and median, 9.020. LSC + : minimum, 7.300; maximum, 12.340; and median, 9.895. f , ATAC-seq pathway enrichment analysis in EV control and NLS1–HK2 NB4 cells. The size of the pie chart slices is proportional to the FDR score, −log 10 (FDR), for each of the gene lists. Blue and pink lines pinpoint to pathways that overlap significantly with the HSC and granulocyte (GRAN) gene lists at FDR < 0.05 according to a Fisher’s exact test. g , Enhanced enrichment of the pathways in NLS1–HK2 and NLS2–HK2 ChIP–seq compared with EV control ChIP–seq in NB4 cells. h , Consensus motif identified by HOMER DNA-binding-motif analysis significantly enriched in NLS1– and NLS2–HK2 peaks at FDR < 0.05. The P value shows significant enrichment of bHLH motifs, determined using a Fisher’s exact test. The boxes with the dashed lines represent sequence similarity overlap. i , Enrichment of the consensus motif CACGTG in sequences of peaks associated with the selected pathways. The hypergeometric P value estimated using the Fisher’s exact test indicate the significance of the enrichment of the motif when compared with random sequences. c – e , Statistical analyses were performed using a two-tailed unpaired Student’s t -test. Data represent the mean ± s.e.m. d , e , In the box-and-whisker plots, the horizontal lines mark the median, the box limits indicate the 25th and 75th percentiles, and the whiskers extend to 1.5× the interquartile range from the 25th and 75th percentiles.
Article Snippet: Antibodies to the following were used: HK2 (Santa Cruz Biotechnology, sc-374091; 1:100), MAX (Santa Cruz Biotechnology, sc-197; 1:200), SPIN1 (Cell Signaling Technology, 89139; 1:100), CTR9 (Cell Signaling Technology, 12619; 1:100) and
Techniques: Mass Spectrometry, Over Expression, Control, ChIP-sequencing, Binding Assay, Sequencing, Two Tailed Test, Whisker Assay